In vitro co-culture of human adipose-derived mesenchymal stem cells and T-lymphocytes from patients with aplastic anemia

doi:10.3969/j.issn.2095-4344.2014.10.020

Abstract

Abstract:

BACKGROUND: Clinical infusion of hematopoietic stem cells and mesenchymal stem cells for treatment of aplastic anemia has been reported.
OBJECTIVE: To investigate the effect of human adipose-derived mesenchymal stems cells on the secretion function of T lymphocytes of aplastic anemia patients.
METHODS: Human adipose-derived mesenchymal stems cells were extracted from healthy human adipose tissues. T-lymphocytes were harvested from peripheral blood of patients with aplastic anemia by density gradient centrifugation. Human adipose-derived mesenchymal stems cells were co-cultured with T-lymphocytes. The levels of interleukin-2, interleukin-4, interleukin-10 and interferon-γ were detected by enzyme linked immunosorbent assay. T-bet and GATA-3 levels were examined by real-time PCR and western blot.
RESULTS AND CONCLUSION: The levels of Th1 type cytokines interferon-γ and interleukin-2 in the co-culture group were significantly lower than those in the T-lymphocyte group (P < 0.05). But the levels of Th2 type cytokines interleukin-4 and interleukin-10 in the co-culture group were significantly higher than those in the T-lymphocyte group (P < 0.05). The T-bet mRNA and protein levels in the co-culture group were significantly lower than those in the T-lymphocyte group, while the GATA-3 mRNA and protein levels were significantly higher in the co-culture group. Human adipose-derived mesenchymal stems cells can mediate an immunoregulation effect on T-lymphocytes of aplastic anemia patients in vitro, which is possibly related with the inhibition of Th1-dominant response due to the disorder of T-bet and GATA-3 gene expression.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, anemia, aplastic, T-lymphocytes

CLC Number:

R394.2

Wang Liang, Xu Min, Zhang Mu-hua, Xing Jian, Zhao Xia, Han Fang, Liu Guo-qiang. In vitro co-culture of human adipose-derived mesenchymal stem cells and T-lymphocytes from patients with aplastic anemia[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(10): 1603-1608.

Figures/Tables 3

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